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You are concerned that Sandy may have a C. difficile infection. When normal microbiota is disturbed, organisms such as C. difficile may thrive. What caused the disturbance in Sandy's normal microbiota in this case?


A) The bacteria causing Sandy's urinary tract infection.
B) The antibiotics Sandy was taking to treat her UTI.
C) Sandy's presence in the hospital.
D) Sandy's watery diarrhea.
E) Dehydration caused by Sandy's watery diarrhea.

F) A) and D)
G) A) and E)

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What conclusion can the scientist in this scenario make from her results?


A) The test bacteria take more than 48 hours to grow in the laboratory when incubated at 37oC.
B) All contaminated water contains high levels of lead and other heavy metals.
C) The amounts of lead used in the experiment killed the bacteria being tested.
D) All experiments should be repeated three times or more.
E) Lead-contaminated water does not contain any bacteria at all.

F) C) and E)
G) All of the above

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You explain to Sandy that her body carries an enormous population of microorganisms, collectively called the normal microbiota. You tell her that this population has a vital role in maintaining her health. Select the FALSE statement regarding the role of the normal microbiota.


A) Normal microbiota prevent disease by competing with pathogenic microbes.
B) Normal microbiota help to degrade foods that the body otherwise could not digest.
C) Normal microbiota synthesize vitamins that the body cannot produce.
D) Normal microbiota produce insulin for controlling blood sugar levels.
E) Normal microbiota likely affects the tendency to lose or gain weight.

F) B) and D)
G) B) and C)

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Viruses are in the domain(s)


A) viridae.
B) eukarya.
C) archaea AND bacteria.
D) bacteria AND viridae.
E) None of the answer choices is correct.

F) All of the above
G) A) and C)

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The outbreak of measles within the last few years is due to


A) mutation of the measles virus.
B) change in the environment.
C) a decline in vaccination of children in the previous years.
D) increase in sensitivity of detection techniques.
E) emergence of novel measles viruses.

F) A) and B)
G) A) and C)

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Viruses may only be grown


A) in sterile growth media.
B) in living cells.
C) at body temperature.
D) in darkness.
E) in liquid broths.

F) C) and D)
G) A) and E)

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Viruses, viroids, and prions are obligate intracellular agents.

A) True
B) False

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The structures present in the hay infusions used in experiments on spontaneous generation that made them difficult to sterilize are


A) chloroplasts.
B) endospores.
C) organelles.
D) toxins.
E) nuclei.

F) A) and E)
G) C) and D)

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Bacteria are present on the body


A) only during disease-causing infections.
B) at all times.
C) only in certain areas.
D) only after intense exercise.
E) only after using public transport.

F) A) and D)
G) C) and E)

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Digestive tract microbiome plays no role in maintaining a person's health.

A) True
B) False

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Microorganisms are involved in all of the following EXCEPT


A) production of medicinal products.
B) converting oxygen to a form useful to plants.
C) food production.
D) pollution cleanup.
E) converting nitrogen to a form useful to plants.

F) C) and D)
G) A) and D)

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Bioremediation refers to


A) rehabilitating wayward bacteria.
B) using bacteria to clean up pollutants.
C) development of new vaccines.
D) monitoring newly discovered disease organisms.
E) destroying organisms causing emerging infectious diseases.

F) A) and B)
G) B) and C)

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Archaea are very similar to bacteria and have rigid cell walls made of peptidoglycan.

A) True
B) False

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Scientists recently cloned Louis Pasteur and put him back to work in a modern lab. He promptly developed a topical gel (used externally) that breaks down proteins. Since he hasn't been around for some time, he's unsure what the best application for his invention might be. Help him out. What pathogenic agent would this gel be most effective and safe at eliminating?


A) Viroids on the surface of agricultural plant tissues.
B) Prions inside the central nervous system of cows.
C) Viruses on the surface of the skin.
D) Bacteria in the intestines of human beings.
E) The fungus that causes infections under people's toenails.

F) B) and E)
G) B) and C)

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Lyme disease is an example of a disease that is due to


A) increased interaction between humans and tick-carrying animals.
B) failure to effectively vaccinate children.
C) a mutation in the human genome.
D) climate change leading to a significantly greater mosquito population.
E) an increase in the number of people travelling to Asia and Africa.

F) B) and C)
G) A) and E)

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All of the statements regarding archaea are true EXCEPT


A) they contain peptidoglycan as part of their cell walls.
B) they reproduce by binary fission.
C) they contain rigid cell walls.
D) they are found as single cells.
E) they often grow in extreme environments.

F) A) and B)
G) B) and C)

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An illness outbreak occurs in New York City birds in the late 1990s. After a lengthy scientific investigation, the Centers for Disease Control (CDC) determine that the agent causing the birds to die is the West Nile virus. Outbreaks of this illness have been observed in several other countries in Asia and the Middle East across the last 50 years, but not in the United States. With this information, what would be the best categorization of this infectious agent/disease?


A) This is a reemerging infection. It is been around for a long time, and it is reappearing in a susceptible population again.
B) This is a nosocomial (hospital-acquired) infection. It is transmitted from animals to human beings in urban environments.
C) This is an emerging infection. It hasn't been around that long, and it has made a jump across continents into a new susceptible population.
D) This is an unimportant infection that not a concern to human beings because it occurs in birds, so there is no need to classify it.
E) This is a chronic infection. It has been around for many years, and it has made a jump across continents into a new susceptible population.

F) C) and D)
G) A) and C)

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Viruses


A) are obligate intracellular parasites.
B) are single-celled organisms.
C) consist of only proteins.
D) are in the domain Archaea.
E) consist only of DNA or RNA.

F) C) and D)
G) A) and C)

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Which of the statements regarding smallpox is TRUE?


A) Smallpox has been eliminated as a naturally occurring infection in human beings.
B) Smallpox still occasionally occurs in developing countries.
C) Smallpox outbreaks sometimes occur in chimpanzee populations.
D) Smallpox has been eliminated by effective vaccination.
E) Smallpox has been eliminated as a naturally occurring infection in human beings, AND has been eliminated by effective vaccination.

F) B) and D)
G) C) and D)

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The scientist usually considered the first to see microorganisms, which he called "animalcules," was ________.


A) Redi
B) van Leeuwenhoek
C) Pasteur
D) Tyndall

E) B) and C)
F) A) and B)

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